01624naa a2200169 a 450000100080000000500110000800800410001910000170006024501440007726000090022152010770023065300290130770000130133670000150134970000200136477300700138416257782004-05-14 2004 bl --- 0-- u #d1 aDECLERCK, S. aDevelopment of extraradical mycelium of Scutellopora reticulata under root-organ culturebspore production and function of auxiliary cells. c2004 aThe development of the extraradical mycelium and auxiliary cells and spore production of Scutellospora reticulata in association with Ri-DNA transformed carrot was followed under root-organ culture conditions. Extraradical mycelium development followed classical lag-exponential-plateau phases, with an additional late decline phase en number of auxiliary cells. Spore production started in parallel with a critical extraradical mycelium biomass produced, continued long after root growth ceased and during the late decline in auxiliary cells were shown to exhibit hyphal re-growth, but not root colonization, either in situ or in vitro. These results showed that root and extraradical mycelium development were intimately associated in a sequence where both grew together during active root growth, followed during root aging by a period in which only the fungus developed. Spore production appeared dependent on a critical extraradical mycelium biomass on the re-allocation of resources from both the intraradical mycelium and the auxiliary cells via the hyphal network. aScutellospora reticulata1 aD'OR, D.1 aBIVORT, C.1 aSOUZA, F. A. de tMycological Research, New Yorkgv.108. n. 1, p. 84-92, jan. 2004.