01657naa a2200217 a 450000100080000000500110000800800410001910000150006024500790007526000090015452010110016365000140117465000210118865300330120965300080124265300080125065300400125870000150129870000170131377301090133016248062006-12-08       2005    bl ---       0--  u    #d1 aPÉREZ, O.  aUpdate on biological nitrogen fixation research on sugarcane in Guatemala.  c2005  aField and laboratory  studies were carried out to (i) quantily the contribution of biological nitrogen fixation (BNF) in several sugarcane varieties grown in Guatemala and (ii) to identify efficient methods of reproducing and inocualting Gluconacetobacter sp. Estimation of BNF contribution was made for 13 sugarcane varieties grown in pots containing 64 kg soil using the 15N isotope-dilution method. The varieties SP79-2233 and PGM 89-968 showed the highest BNF potential, being able to obtain 53 and 60% respectively of their nitrogen (N) uptake through BNF. Different liquid and solid culture media for growing Gluconacetobacter and four different methods of inoculation of Gluconacetabocter; either alone or in combination with Herbaspirillum sp; were evaluated. Gluconacetabocter reproduced more efficiently in liquid growing media when compared with solid media. All four methods of inoculation were equally effective as indicated by a higher biomass production compared to the uninoculated control.  asugarcane  aCana de Açúcar  aBiological nitrogen fixation  aBNF  aFBN  aFixação biológica de nitrogênio1 aOVALLE, W.1 aURQUIAGA, S.  tSugar cane International; The Journal of Cane Agriculture, Bucksgv. 23, n. 2, p. 19-22, mar./apr. 2005.