01876naa a2200193 a 450000100080000000500110000800800410001910000170006024500690007726000090014652013470015570000160150270000250151870000150154370000180155870000180157670000180159477300700161216211952001-03-23       2000    bl ---       0--  u    #d1 aGYORGYEY, J.  aAnalysis of Medicado truncatula nodule expressed sequences tags.  c2000  aSystematic sequencing of expressed sequence tags (ESTs) can give a global picture of the assembly of genes involved in the development and function of organs. Indeterminate nodules representing different stages of the developmental program are especially suited to the study of organogenesis. With the vector hybriZAP, a cDNA library was constructed from emerging nodules of Medicago truncatula induced by Sinorhizobium meliloti. The 5'ends of 389 cDNA clones were sequenced, then these ESTs were analyzed both by sequence homology search and by studying their expression in roots and nodules. Two hundred fifty-six ESTs exhibited significant similarities to characterized data base entries and 40 of them represented 26 nodulin genes, while 133 had no similarity to sequences with known function. Only 60 out of the 389 cDNA clones corresponded to previously submitted M. truncatula EST sequences. For 117 cDNAs, reverse Northern (RNA) hybridization with root and nodule RNA probes revealed enhanced expression in the nodule, 48 clones are likely to code for novel nodulins, 33 cDNAS are clones of already known nodulingenes, and 36 clones exhibit similarity to other characterized genes. Thus, systematic analysis of the EST sequences and their expression patterns in a powerful was to identify nodule-specific and nodulation-related genes.1 aVAUBERT, D.1 aJIMENEZ-ZURDO, J. I.1 aCHARON, C.1 aTROUSSARD, L.1 aKONDOROSI, A.1 aKONDOROSI, E.  tMolecular Plant-Microbe Interactionsgv. 13, n. 1, p 62-71, 2000.