01791naa a2200253 a 450000100080000000500110000800800410001910000160006024501190007626000090019552010090020465000170121365000160123065000170124665000140126365000120127765000120128965000140130165300140131570000150132970000150134470000190135977301590137816209671999-01-25       1997    bl uuuu     u00u1 u    #d1 aMARTINS, A.  aEffect of the co-inoculation of plant-growth promoting rhizobacteria on the ultrastructure of common bean nodules.  c1997  aRhizobacteria have been used as biocontrol agent of many soil-borne pathogens as well as phytohormone plant - producers of several plants including legumes and promoting increase of root area and nodulation. In this work we evaluated the effect of a fluorescent Pseudomonas sp. strain, isolated from the rhizosphere of common bean, on the structure of nodules formed by strains of R. leg. bv. phaseoli and R. tropici. Nodules formed solely by rhizobia strains were pink while they appeared greenished when rhizobacteria were coinoculated. The presence of the rhizobacteria was confirmed by plate counting and fluorescence production when nodules were exposed to UV light. Light microscopy sections from nodules originated from the coinoculation with rhizobacteria showeda small amount of infected cells. These cells were not fully occupied by the bacteroids and in addition they were not enclosed in a membrane envelope (peribacteroid membrane). In general, the bacteroids appeared free in the host cell.  akidney beans  arhizosphere  aroot nodules  aBactéria  aFeijão  aNódulo  aRizosfera  aBacterias1 aKIMURA, O.1 aGOI, S. R.1 aBALDANI, J. I.  tIn: INTERNATIONAL CONGRESS ON NITROGEN FIXATION, 11., jul. 1997, Paris. Abstracts... Paris: Institut Pasteur; INRA; CNRS; CEA; ORSTOM; CIRAD, 1997. p.108.