02964naa a2200313 a 450000100080000000500110000800800410001910000220006024501080008226000090019052019330019965000150213265000140214765000210216165000230218265000260220565300080223165300080223965300360224765300290228365300150231270000170232770000160234470000200236070000190238070000230239970000190242277302090244116209461997-12-01       1996    bl uuuu     u00u1 u    #d1 aCOSTA LIMA, M. A.  aDetection of sugarcane (Saccharum officinarum L.) genes induced by endophytic nitrogen-fixing bacteria.  c1996  aNitrogen is an essential element for all living organisms,beig used for protcin and nucleic acids biosynthesis. it is assimilated by plants and animals as ammoniom(NH4) or nitrate (NO3).atmospheric nitrogen(N2)despite its abundance, cannot be utilized directly.biologic nitrogen fixation (BNF) is the process by wich atmosphcric nitrogen becone available and consiste basically in nitrogen reduction,in process caralyzed by enzymes. only a few species of bacteria and algae are able to do this process. endophytic nitrongen-fixing bacteria as acetobacter and herbaspirillum were foud in sugarcane tissues.in this symbiotic association, BNF occurs withont induction of nodule formation, in a system that differs from rhizobium-leguminoscre interaction. in this system, litle is known about plant-bacteria interaction of BNF in distinc sugarcane genotypes indicates that plant genetic factors are contributing to the efficiency of the process. the isolation and characterization of plant genes induced during the processes of recognition, colonization and nitrogen fixation by the bacteria wil provid tools for future genetic manipulation to obtain high rales of BNF. In order to study the role of the plant in this new system of symbiotic association we have useda modification of the differential disply technique to detect and isolate cDNAs corresponding to transcripts expressed in sugarcane plants infected wit Acetobacter diazotrophichs and Herbaspirillum spp..plants were propagated and infected in vitro. In a preliminary screenig, eight  different random primers were selected to amplify cDNAs present only in infected plants. The differentially expressed bands obtained were eletud and reaplifiend using the same primers. Southern blot hybridizations using total cDNA-obtained from infected plants as probe are beig performed to confim differential expression. The induced genes will be cloned and further characterized.  aendophytes  aBactéria  aCana de Açúcar  aParasito de Planta  aSaccharum Officinarum  aBNF  aFBN  aFixacao biologica de nitrogenio  aNitrogen fixing bacteria  aSugar cane1 aVILAR, C. V.1 aREIS, V. M.1 aOLIVARES, F. L.1 aBALDANI, J. I.1 aFERREIRA, P. C. G.1 aHEMERLY, A. S.  tIn: REUNIAO ANUAL DA SOCIEDADE BRASILEIRA DE BIOQUIMICA E BIOLOGIA MOLECULAR, 25., maio 1996, Caxambu. Resumos... Caxambu: Sociedade Brasileira de Bioquimica e Biologia Molecular, 1996. p.42. Resumo E-74.