01900naa a2200265 a 450000100080000000500110000800800410001910000160006024500750007626000090015152011350016065000160129565000240131165000210133565000160135665300150137265300170138765300170140465300270142165300190144865300350146765300160150270000230151877300930154115802302010-01-13 2009 bl uuuu u00u1 u #d1 aMELO, D. F. aIdeal culture conditions for Dicyma pulvinata conidia mass production. c2009 aThe objective of this work was to set up ideal conditions for conidia mass production of Dicyma pulvinata. Four isolates were compared in terms of their growth and conidia production on various substrates (grains of parboiled rice, common rice, maize and wheat, besides chipped maize and rice husk), temperatures (19, 22, 25, 28 and 31oC), growth containers (aluminum trays, polypropylene bags and Erlenmeyers) and light regimes (continuous darkness, 6 and 12 hours of light/darkness, and continuous light). Temperature effects on conidia germination capacity were also evaluated. The experiments were done in randomized complete block designs, in factorial arrangements (isolates x treatments ? substrates, containers, temperatures and light regimes), with four replicates. In general, parboiled rice and polypropylene bags provided the best development of the fungus. Complete darkness and 6 hours of light increased mycelial growth, whereas continuous light favored sporulation. All tested temperatures favored the cultures of the fungus, except 31oC. Temperatures between 19 and 25oC ensure spore germination of more than 76%. aLeaf blight aControle Biológico aMicrocyclus Ulei aSeringueira aBiocontrol aBiofungicida aBiofungicide aFungo Dicyma pulvinata aMal-das-folhas aProdução massal de conídios aRubber tree1 aMELLO, S. C. M. de tPesquisa Agropecuária Brasileira, Brasília, DFgv. 44, n. 10, p. 1232-1238, out. 2009.