01843naa a2200277 a 450000100080000000500110000800800410001902400320006010000200009224501570011226000090026952009890027865000210126765000240128865000230131265300260133570000200136170000220138170000190140370000150142270000190143770000180145670000130147470000170148777300610150415784772010-01-13       2009    bl ---       0--  u    #d7 a10.1007/s12686009908012DOI1 aSOUSA, A. C. B.  aMicrosatellite markers in tropical legume (Centrosema pubescens Benth)bdevelopment, characterization, and cross-species amplification in Centrosema sp.  c2009  aCentrosema pubescens Benth is a forage legme widespread in tropical America. Twenty-six poly-morphic microsatellite markers were isolated and characterized in 15 genotypes of C. pubescens from the Cerrados Research Center Germsplams Bank of te Brazilian Agricultural Research Comporation (Embrapa). The number of alleles observed for each locus ranged from 2 to 5, with an average of 3 alleles per locus. The polymorphism information content (PIC) varied between 0.39 and 0.86 (average 0.57) and the discriminating power (D) ranged from 0.45 to 0.98 (average 0.68). The observed heterozygosity (Ho) and the expected heterozygosity (He) werw 0.01-0.81 and 0.10-0.86, respectively. A cross-amplification test in 11 Centrosema spécies suggest pottntial transferability of these microsatellites. The data indicated that the polymorphic microsatellite markers developed in this work should be useful for assessing genetic diversity in further breeding programs and germplasm conservation.  aGenetica vegetal  aLeguminosa tropical  aMarcador genético  aDiversidade genética1 aCARVALHO, M. A.1 aBOAVENTURA, L. R.1 aSFORÇA, D. A.1 aCAMPOS, T.1 aCANCADO, L. J.1 aZUCCHI, M. I.1 aJANK, L.1 aSOUZA, A. P.  tConservation Genetics Resourcesgv. 1, p. 347-352, 2009.