02277nam a2200253 a 450000100080000000500110000800800410001910000250006024500830008526000690016850001050023752014680034265000220181065000100183265000210184265000220186365300220188570000190190770000200192670000200194670000140196670000230198070000200200315582352010-02-24       2009    bl uuuu  j   00u1 u    #d1 aANDRADE, S. R. M. de  aUses of alternative antibiotics to control bacterium growth in mango explants.  aActa Horticulturae, Leuven, n. 820, p. 541-546, abr. 2009.c2009  aEdição do Proceedings of the 8 International Mango Symposium, Sun City, South Africa, Abril, 2009.  aBacterias, if they are not eliminated from the explant, compete for nutrients from the culture medium and also eliminate toxins in the medium modifying the normal development of the explant. The decontamination consists of microorganisms elimination, however preserving plant explant to be regenerated. The objsctive of this work was to develop a protocol to control and endogenous bacterium of mango internodal segments for the introduction on plant tissue culture medium. The explants had been submitted to treatments with ethanol, sodium hypoclorite and benomyl, for surface decontamination. The evaluation demonstrated that the bacterium is gram positive and resistant to the antibiotics rifampycin (300 mg.L-1), ampicylin (150 mg.L-1), tetracyclin (150 mg.L-1), chloramphenicol (150 mg.L-1), carbenicyllin (150 mg.L-1) and streptomycin (300 mg.L-1). However, the results demonstrated that the combination of sulphametaxazole + trimetroprim (80 mg.L-1 + 16 mg.L-1) or sulfamethoxazole (80 mg. L-1) alone showed satisfatory result on the control of this endogenous bacterium, while trimethoprim (16 mg.L.-1) did not work alone. Preliminary studies demonstrated that inclusion of copper sulphate (25 and 50 mg.L-1) into the nutritive medium controlled the endogenous fungi, however did not control of the bacterium growth. Medium containing 1% of propolis extract had also contolled the bacterium development, however the explants growth had also been affected.  aCultura de Tecido  aManga  aMangifera Indica  aMicropropagação  aDescontaminação1 aFALEIRO, F. G.1 aTEIXEIRA, J. B.1 aPINTO, A. C. Q.1 aRADEL, G.1 aCORDEIRO, M. C. R.1 aRAMOS, V. H. V.