03488naa a2200385 a 450000100080000000500110000800800410001910000230006024501630008326000090024652023510025565000110260665000410261765000350265865000180269365000100271165000190272165000120274065000190275265000110277165300310278265300110281365300090282465300160283370000170284970000250286670000200289170000200291170000200293170000200295170000200297170000220299170000260301377300630303915322932023-07-10       2009    bl uuuu     u00u1 u    #d1 aPAULA, N. R. de O.  aProfile of the Caprine arthritis-encephalitis virus (CAEV) in blood, semen from bucks naturally and experimentally infected in the Semi-arid region of Brazil.  c2009  aAbstract: The aim of this study was to report the chronology of Caprine arthritis-encephalitis virus elimination and compare the blood and semen viral profiles of animals naturally and experimentally infected by SRLV raised in the semi-arid region of Brazil. The experiment was carried out at the Brazilian Center for Goat Research (Embrapa). Nine bucks were selected, four naturally infected by CAEV and five animals proven negative thatwere inoculated with the goat lentivirus (CAEV-Cork strain). Every week the animals were submitted to semen collection using an artificial vagina. The blood was collected by puncturing the jugular vein with tubes containing EDTA, 7 days after inoculation (experimentally infected group) or at the start of the experiment (naturally infectedgroup) and then at every 30days. The genomic viral DNA was extracted from semen and blood and then Nested-PCR was applied. An agar gel microimmunodiffusionwas performed to detect anti-CAEV antibodies. The resultswere described in percentage and analyzed by the Chi square test (P &lt 0.05). The presence of anti-CAEV antibodies was detected in the 16th week after inoculation that characterized the seroconversion from four of the five naturally infected goat bucks (80%). The fifth reproducer presented late seroconversion, totaling 32 weeks post-inoculation. A quantity was observed in the total of samples collected of 12.50 and 17.14% positive results in the blood and 10.98 and 11.25% positive results in the semen of the naturally and experimentally infected animals, respectively, and there was no statistical difference. No statistically significant differences were observed regarding the presence of proviral DNA in the blood and semen of the naturally and experimentally infected animals. A viral elimination pattern was not identified during the assessment period, but the presence of proviral DNA was shown at shorter intervals after the 18th week and the 22nd week, for the experimentally and naturally infected bucks, respectively. Therefore, recently infected goats in the period prior to seroconversion eliminated small ruminant lentivirus proviralDNA in the semen and are important sources of infection that should be considered in a control program of this lentivirus, and the Nested-PCR technique is a relevant tool to select virus-free ejaculates.  aBrazil  aCaprine arthritis encephalitis virus  aCaprine arthritis-encephalitis  aGoat diseases  aGoats  aSemiarid zones  aCaprino  aDoença Animal  aSêmen  aArtrite encefalite caprina  aBrasil  aCAEV  aSemi-árido1 aANDRIOLI, A.1 aCARDOSO, J. de F. S.1 aPINHEIRO, R. R.1 aSOUSA, F. M. L.1 aSOUZA, K. C. de1 aALVES, F. S. F.1 aCAMPELLO, C. C.1 aRICARTE, A. R. F.1 aTEIXEIRA, M. F. da S.  tSmall Ruminant Researchgv. 85, n. 1, p. 27-33, Jul. 2009.