02439naa a2200385 a 450000100080000000500110000800800410001902400420006010000250010224501200012726000090024752012880025665000200154465000190156465000200158365000220160365000470162565000080167265000230168065000230170365000270172665000250175365300100177865300190178865300250180765300220183265300240185465300270187865300260190565300250193170000160195670000150197270000200198777300460200715303592023-08-28 2003 bl uuuu u00u1 u #d7 a10.1046/j.1439-0523.2003.00830.x2DOI1 aARAÚJO, M. R. A. de aRandom amplified polymorphic DNA variation within and among meadow bromegrass progeny types.h[electronic resource] c2003 aTen meadow bromegrass genotypes tested as half-sib (polycross, PX; open-pollinated, OP) and selfed (S1) progenies were surveyed using random amplified polymorphic DNA. Fourteen primers, which produced 32 markers, were selected to determine the genotypes of 360 individuals from the three progeny tests. Analysis of molecular varian? was performed in each progeny test, and genetic distances between genotypes and progeny types were determined. Among genotype genetic variation in half-sib (PX and OP) progenies was similar. Genetic variation among genotypes for S1 progenies (30.5%) was about twice that round in the half-sib progenies. Variation between individual S1 progenies ranged from 15.7% to 50.1%, while in the half-sib progenies, the range was 6.9- 24.1 %. Based on average distan?s between progeny types for a given genotype, OP and PX were closer to each other than to the S1. An analysis of variance of the molecular marker frequency occurren? was performed for 12 plants within each progeny type of each genotype. Marker frequencies, expressed in percentages, ranged from 10.7% to 84.3%. No significant differences were round for genotype and progeny type-genotype interaction suggesting that ail genotypes behave in a similar manner across the different progeny types. aBromus riparius aForage grasses aGenetic markers aGenetic variation aRandom amplified polymorphic DNA technique aDNA aMarcador Genético aMarcador Molecular aPolimorfismo Genético aVariação Genética aAMOVA aBromus riparus aDistância genética aGenetic distances aGenetic relatedness aGramíneas forrageiras aRAPD marker variation aVariação molecular1 aCOULMAN, B.1 aSOMERS, D.1 aFERDINANDEZ, Y. tPlant Breedinggv. 122, p. 416-420, 2003.