03505naa a2200361 a 450000100080000000500110000800800410001902400410006010000170010124500730011826000090019152024510020065000240265165000280267565000410270365000100274465000240275465000150277865000270279365000230282065000120284365000240285565000110287965000280289065300230291865300090294165300190295070000220296970000220299170000200301370000180303377300920305115302302021-01-27       2006    bl uuuu     u00u1 u    #d7 a10.1590/S0100-204X20060008000152DOI1 aANDRIOLI, A.  aFatores de risco na transmissão do lentivírus caprino pelo sêmen.  c2006  aResumo: 0 objetivo deste trabalho foi avaliar a presença do DNA pro-viral do lentivirus caprino (LVC) em ejaculados de machos infectados naturalmente, e verificar a influencia da lavagem do semen e da presença de  inflamaçao testicular sobre a carga viral. Foram realizadas oito coletas de semen de sete reprodutores soropositivos para o LVC: quatro antes dos animais sofrerem dano testicular e quatro depois. Entre as coletas realizadas na mesma semana, em uma, 0 ejaculado era lavado, para retirada do plasma seminal, e na outra, nao. 0 DNA pro-viral do LVC foi identificado pela reaçao ern cadeia da polimerase Nested (PCR Nested), e pelo isolarnento viral. 0 virus foi isolado em 7,1 % das amostras. A PCR identificou 0 DNA pro-viral ern 35,7% do total das amostras: 17,9% nas amostras lavadas e 53,6% das amostras de semen integrais. 0 dano ao testiculo permite maior fluxo do virus para o semen, pois antes do dano, 21,4% das amostras foram positivas e pos-dano, 50%. A transmissao do LVC pelo semen de reprodutores caprinos e potencializada pela presença de inflamaçoes testiculares e pelo fato de o semen criopreservado conter o LVC na forma infectante.  [Risk factors in caprine lentivirus transmission through semen]. Abstract: The objective of this work was to evaluate the presence of the DNA provirus of the caprine lentivirus (LVC) in ejaculates of naturally infected males, and to verify the influence of the wash of the semen as well as the presence of testicle inflammation on the viral load. Eight semen collections of seven soropositive reproducers were accomplished, four before testicle injury and four after injury. Amongst the collections carried out at the same week, in one the ejaculate was washed, to withdraw the plasma seminal, and in the other it was not. The provirus DNA was identified both by Nested polymerase chain reaction technique (Nested PCR) and by the viral isolation. The virus was isolated in 7.1% of the samples. The PCR identified the provirus DNA in 35.7% of all samples, 17.9% in the washed samples and 53.6% of the integral semen samples. The injury of the testicle tends to greater flow of virus for the semen, therefore, before injury, 21.4% of the samples were positive and after-injury, 50%. Risk of transmission of the LVC by semen of goat reproducers is strengthened by the presence of testicle inflammations and the fact that the criopreserved semen contains the LVC in infecting form.  aAnimal reproduction  aArtificial insemination  aCaprine arthritis encephalitis virus  aGoats  aInfectious diseases  aLentivirus  aReproductive disorders  aVirus transmission  aCaprino  aReprodução animal  aSêmen  aTransmissão de doença  aArtrite Encefalite  aCAEV  aInfected bucks1 aGOUVEIA, A. M. G.1 aMARTINS, A. de S.1 aPINHEIRO, R. R.1 aSANTOS, D. O.  tPesquisa Agropecuária Brasileira, Brasília, DFgv. 41, n. 8, p. 1313-1319, ago. 2006.