02426naa a2200409 a 450000100080000000500110000800800410001902400290006010000140008924501630010326000090026652012600027565000210153565000200155665000130157665000200158965000330160965000130164265000170165565000140167265000170168665000100170365000190171365000180173265300260175065300170177665300080179365300190180165300080182065300240182865300190185270000170187170000150188870000140190370000160191777300830193315300392023-08-28       2001    bl uuuu     u00u1 u    #d7 a10.1051/rnd:20011272DOI1 aLEONI, G.  aSheep embryos derived from FSH/eCG treatment have a lower in vitro viability after vitrification than those derived from FSH treatment.h[electronic resource]  c2001  aAbstract: In the non breeding period, the effect of two superovulatory treatments (eCG/FSH in single dose or FSH alone in four decreasing doses) on the production of embryo quality following in vitro viability after vitrification procedures was investigated using forty-four adult Sarda breed ewes. In sheep treated with eCG/FSH, the mean number of corpora lutea was significantly (P &lt 0.05) higher (11.8+/-4.0 vs. 8.05+/-3.8), although the recovery rate was significantly (P &lt 0.01) lower (74.6 vs. 59.9) than with FSH alone. After vitrification (ethylene glycol and glycerol) was repeated three times, the rates of re-expansion at first and second warming were significantly (P &lt 0.01) higher in embryos derived from FSH alone than in those with both gonadotrophins (94.9 and 41.9 vs. 72.8 and 18.6) and after the last vitrification the hatched blastocyst rates were 22.5 and 7.6. After differential stain, blastocysts derived from FSH alone showed a mean number of cells significantly higher than blastocysts from eCG/FSH (184.2 vs. 157.7). It was concluded that superovulatory treatment with eCG/FSH may increase the ovarian responses compared with FSH alone, but these embryos showed a reduction in viability rates after repeated vitrification.  aCryopreservation  aEmbryo transfer  aEthylene  aEthylene glycol  aFollicle-stimulating hormone  aGlycerol  aPharmacology  aPregnancy  aReproduction  aSheep  aSuperovulation  aVitrification  aCryoprotective agents  aDrug effects  aeCG  aEmbryo quality  aFSH  aIn Vitro Techniques  aVitrificação1 aBOGLIOLO, L.1 aPINTUS, P.1 aLEDDA, S.1 aNAITANA, S.  tReproduction, Nutrition, Developmentgv. 41, n. 3, p. 239-246, May./Jun. 2001.