01940naa a2200253 a 450000100080000000500110000800800410001910000180006024501410007826000090021952012050022865000280143365000170146165000100147865000180148865000160150665000290152265000100155165000240156165000110158565300130159670000210160977300560163015294472025-06-18       1976    bl uuuu     u00u1 u    #d1 aWATSON, P. F.  aArtificial insemination of sheepbthe effect of semen diluents containing egg yolk on the fertility of ram semen.h[electronic resource]  c1976  aAbstract: PIP: Results from an artificial insemination (AI) experiment revealing the effect of semen dilutents containing egg yolk on the fertility of ram semen are presented. Ram semen was diluted 30-fold in buffered glucose-saline solution containing . 375, 1.5, or 6% v/v egg yolk and a portion of each was used soon for the AI of ewes or was incubated at 35 degrees C for 1 hour prior to AI. Some of the semen collection was used undiluted for AI of 10(8) spermatozoa/dose. All diluted samples were reconcentrated by centrifugation so that each dose was 10(8) spermatozoa in a volume of 100 mcl. 1146 ewes were inseminated. Fertility was assessed from 28 to 45 day nonreturns to estrus and nonreturn rates (NRRs) were expressed as percentages for the various treatments. Undiluted semen (controls) revealed 69%, semen used soon after dilution, .375% yolk in dilutent 58%, 1.5% yolk 50%, 6% yolk 42%; diluted semen incubated for 1 hour before use, .375% yolk 49%, 1.5% yolk 51%, and 6% yolk 39%. NRR was significantly depressed by dilution (p less than .001) and by increasing amounts of egg yolk (p less than .01) in the dilutent. Incubation of diluted semen before AI caused a small fall in NRR.  aArtificial insemination  aReproduction  aSheep  aConservação  aGema de Ovo  aInseminação Artificial  aOvino  aReprodução Animal  aSêmen  aDiluente1 aMARTIN, I. C. A.  tTheriogenologygv. 6, n. 5, p. 559-562, No.v, 1976.