01915naa a2200289 a 450000100080000000500110000800800410001902400540006010000210011424501790013526000090031452010170032365000210134065000280136165000170138965000100140665000160141665000160143265000290144865000100147765000240148765000110151165300130152265300190153570000180155477300530157215294432023-08-25       1976    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1016/0093-691X(76)90155-22DOI1 aMARTIN, I. C. A.  aArtificial insemination of sheepbeffects on fertility of number of spermatozoa inseminated and of storage of diluted semen for up to 18 hours at 5°C.h[electronic resource]  c1976  aAbstract: Ram semen was prepared in a buffered glucose-saline solution containing 3% (v/v) egg yolk so that insemination doses of 25 or 100 million spermatozoa in volumes of 50 or 250 ?l could be given per ewe at artificial insemination (AI). Fertility was significantly reduced by dilution and, within the treatments of diluted semen, significantly higher lambing rates followed the use of doses of 100 million spermatozoa. The volume of the AI dose had no significant effect on fertility. Of 945 inseminations performed using diluted semen, 388 were with samples that had been cooled to 5°C and stored chilled for 5 or 18 hr. The mean lambing result of 40% for freshly diluted semen was significantly higher than 31.6% and 30.2% for samples stored chilled for 5 and 18 hr respectively. Ewes inseminated with doses of chilled semen containing 25 million spermatozoa had a low lambing rate of 21.3%. The presence of 7.5% glycerol (v/v) in the diluent did not significantly affect the fertility of chilled semen.  aAnimal fertility  aArtificial insemination  aReproduction  aSheep  aSpermatozoa  aFertilidade  aInseminação Artificial  aOvino  aReprodução Animal  aSêmen  aDiluente  aSemen diluents1 aWATSON, P. F.  tTheriogenologygv. 5, n. 1, p. 29-35, Jan. 1976.