01466naa a2200325 a 450000100080000000500110000800800410001902400540006010000200011424501100013426000090024452005070025365000210076065000260078165000140080765000390082165000150086065000190087565000140089465000190090865000170092765000110094465300180095565300200097365300130099370000200100670000160102670000290104277300690107115293332023-08-25       1993    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1016/0166-0934(93)90063-W2DOI1 aARITA, G. M. M.  aBluetongue virusbproduction and study of viral antigen for serological diagnosis.h[electronic resource]  c1993  aA soluble antigen, produced from the culture supernatant of VERO cells infected with bluetongue virus serotype 4 (BTV-S4) and concentrated by sequential ultrafiltration with membranes with cut-off values 103 and 25 x 103 NMWP, showed complete identity to standard antigens when compared by agar gel immunodiffusion (AGID) and SDS-PAGE profiles, revealing that the main protein component responsible for the AGID reaction has a molecular weight of about 60 kDa corresponding probably to the NS1 protein.  aBluetongue virus  aImmunodiffusion tests  aOrbivirus  aPolyacrylamide gel electrophoresis  aReoviridae  aSheep diseases  aAnticorpo  aDoença Animal  aLíngua Azul  aVírus  aImunodifusão  aSoluble antigen  aSorotipo1 aGATTI, M. S. V.1 aDEAK, J. G.1 aPESTANA DE CASTRO, A. F.  tJournal of Virological Methodsgv. 44, n. 2/3, p. 281-286, 1993.