01825naa a2200409 a 450000100080000000500110000800800410001902400410006010000220010124501450012326000090026852006470027765000230092465000170094765000260096465000230099065000200101365000230103365000150105665000210107165000180109265000210111065000130113165000190114465000140116365000140117765000110119165300140120265300310121665300160124765300150126370000160127870000180129470000180131270000190133077300660134915241192024-01-09       1992    bl uuuu     u00u1 u    #d7 a10.1128/iai.60.7.2900-2905.19922DOI1 aHODGSON, A. L. M.  aRational attenuation of Corynebacterium pseudotuberculosisbpotential cheesy gland vaccine and live delivery vehicle.h[electronic resource]  c1992  aThe phospholipase D (PLD) gene (pld) has been deleted from the Corynebacterium pseudotuberculosis chromosome by using site-specific mutagenesis. Sheep infection trials indicate that the PLD-negative C. pseudotuberculosis strain (Toxminus) is incapable of inducing caseous lymphadentis (cheesy gland) even at doses two logs higher than that at which the wild-type strain produces the disease. This clearly establishes PLD as a major C. pseudotuberculosis virulence factor. Vaccination of sheep with live Toxminus C. pseudotuberculosis elicits strong humoral and cell-mediated immune responses and protects the animals from wild-type challenge.  aAntibody formation  aBiosynthesis  aCaseous lymphadenitis  aChromosome mapping  aDisease control  aDisease prevention  aImmunology  aInterferon-gamma  aLive vaccines  aPassive immunity  aPlasmids  aSheep diseases  aVirulence  aGenética  aVacina  aAtenuacao  aDose-Response Relationship  aLinfadenite  aVetor vivo1 aKRYWULT, J.1 aCORNER, L. A.1 aROTHEL, J. S.1 aRADFORD, A. J.  tInfection and Immunitygv. 60, n. 7, p. 2900-2905, Jul. 1992.