02040naa a2200409 a 450000100080000000500110000800800410001902400360006010000240009624501060012026000090022652009020023565000230113765000170116065000310117765000170120865000170122565000170124265000170125965000080127665000100128465300210129465300380131565300130135365300140136665300120138065300170139265300090140970000230141870000150144170000200145670000180147670000210149470000170151570000190153277300790155115240562023-07-13       1994    bl uuuu     u00u1 u    #d7 a10.1093/infdis/170.4.1027.2DOI1 aMARSHALL III, W. F.  aDetection of Borrelia burgdorferi DNA museum specimens of Peromyscus leucopus.h[electronic resource]  c1994  aAbstract: To determine whether Borrelia burgdorferi was enzootic within the United States at the beginning of the 20th century, ear skin samples taken from museum specimens of the white-footed mouse (Peromyscus leucopus) were examined for evidence of spirochetal DNA. In total, 280 samples from mice collected between 1870 and 1919 were analyzed by a nested polymerase chain reaction protocol. Of these, 2 specimens from the vicinity of Dennis, Massachusetts, during 1894 were reproducibly positive for B. burgdorferi OspA sequences. The remaining 278, representing both currently endemic and nonendemic sites, were negative for spirochetal DNA. These studies suggest that the agent of Lyme disease was present in a suitable reservoir host in the United States before the turn of the century and provide evidence against a hypothesis of recent introduction of this zoonotic agent to North America.  aBacterial vaccines  aBiosynthesis  aBorrelia burgdorferi group  aEpidemiology  aLipoproteins  aLyme disease  aMicrobiology  aDNA  aVetor  aAntigens surface  aBacterial Outer Membrane Proteins  aDeteccao  aDNA Viral  aMuseums  aOspA protein  aSkin1 aTELFORD III, S. R.1 aRYS, P. N.1 aRUTLEDGE, B. J.1 aMATHIESEN, D.1 aMALAWISTA, S. E.1 aSPIELMAN, A.1 aPERSING, D. H.  tThe Journal of Infections Diseasesgv. 170, n. 4, p. 1027-1032, Oct. 1994.