01436naa a2200265 a 450000100080000000500110000800800410001910000230006024500880008326000090017152006860018065000210086665000210088765000140090865000100092265300130093265300190094565300270096465300200099165300210101170000310103270000180106370000200108177300690110115238852023-07-13       1995    bl uuuu     u00u1 u    #d1 aOLIVEIRA, S. A. de  aIn vitro propagation of Cereus Peruvianus mill. (Cactaceae).h[electronic resource]  c1995  aAbstract: Cereus peruvianus seedlings were used as a source of stem explants to determine the effective conditions for inducing and maintaining callus tissues in a state of rapid growth, as well as to obtain plants regenerated from callus cultures. Factorial combinations of 2,4-dichlorophenoxyacetic acid (2,4-D) and kinetin in MS medium were tested, and we concluded that the 18.1 uM 2,4-D and 18.6 or 27.9 1M kinetin combinations were suitable for callus induction. The cactus shoots were produced from the friable callus; root elongation occurred within 2 wk in medium without 2,4-D and with 18.6 1iM kinetin. This method can be used to rapidly produce many C. peruvianus plan.  aIn vitro culture  aMicropropagation  aCactaceae  aCacto  aCactacea  aFriable callus  aSeedling stem explants  aShoot formation  aSucculent plants1 aMACHADO, M. de F. P. da S.1 aPRIOLI, A. J.1 aMANGOLIN, C. A.  tIn Vitro Cell. Development Biologygv. 31, n. 1, p. 47-50, 1995.