02817naa a2200373 a 450000100080000000500110000800800410001902400490006010000180010924500920012726000090021952018220022865000220205065000240207265000180209665000150211465000140212965000140214365000170215765000190217465000100219365000110220365000190221465000150223365000100224865000190225865000170227765000110229470000160230570000220232170000190234370000190236277300620238115230812023-05-31       1975    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.2527/jas1975.411144x2DOI1 aPEXTON, J. E.  aProstaglandins F in uterine tissue and venous plasma of ewes with intrauterine devices.  c1975  aLevels of prostaglandins F (PGF) in uterine tissues and in uterine and peripheral plasma from ewes with and without IUD's were examined. Mature ewes were laparotomized on day 2 of the estrous cycle. Fifteen ewes with a corpus luteum on only one ovary were assigned at random to either a treated group (10 ewes) which received an intrauterine device (IUD) in each uterine horn or to a control group (five ewes). Twelve ewes had a corpus luteum on each ovary and received at random an IUD in one uterine horn (eight ewes) or a sham operation (four ewes). Blood was collected from a major vein of each uterine horn on day 5. Uteri were removed and the uterine horns were separated and dissected into caruncular and intercaruncular endometrium and myometrium. Prostaglandins F were extracted with ethyl acetate, separated by column chromatography on silicic acid and quantified by radioimmunoassay. An IUD in one or in both uterine horns resulted in higher concentrations (ng/ml) of PGF in uterine venous plasma from both horns (6.4 + 2.2 to 9.3 + 2.7) than in controls (.5 + .1 to .6 -+ .2). PGF (ng/g) in uterine tissue from ewes which had an IUD in each uterine horn was considerably higher in caruncular (382 + 42), intercaruncular (641 -+ 95) and myometrial tissue (82 + 9) than in controls (199 + 49, 240 -+ 72 and 50 -+ 11 in caruncular and intercaruncular endometrium and myometrium, respectively). In bilaterally-ovulating ewes with an IUD in one uterine horn, caruncular endometrium from those horns consistently contained more PGF (293 + 23 ng/g) than caruncular endometrium from uterine horns not containing an IUD (149 + 21) or from control ewes (130 + 24). The concentration of PGF in jugular plasma from IUD-bearing ewes (.4 + .1) was not different from that measured in plasma from control ewes (.4 + .1).  aAnimal physiology  aAnimal reproduction  aCorpus luteum  aMetabolism  aOvulation  aPregnancy  aProgesterone  aProstaglandins  aSheep  aUterus  aEndocrinologia  aFisiologia  aOvino  aProstaglandina  aReprodução  aÚtero1 aFORD, S. P.1 aWILSON JUNIOR, L.1 aBUTCHER, R. L.1 aINSKEEP, E. K.  tJournal of Animal Sciencegv. 41, n. 1, p. 144-153, 1975.