01629naa a2200229 a 450000100080000000500110000800800410001910000200006024501650008026000090024552008870025465000150114165000140115665000230117065000190119365000200121265000150123265000220124765000230126970000190129277300880131115229442024-04-08       1975    bl uuuu     u00u1 u    #d1 aJONES, D. I. H.  aThe effect of pepsin pretreatment of herbage on the prediction of dry matter digestibility from solubility in fungal cellulase solutions.h[electronic resource]  c1975  aAbstract: Cellulase preparations from different fungi differed markedly in their ability to solubilise herbage and cellulose; T. viride cellulase was the most active, solubilising 70% of cellulose paper in 24 h. The correlation of cellulase solubility with the in vivo and in vitro dry matter digestibility of grasses, and with the in vitro digestibility of legumes was markedly improved by pretreatment of the herbage with acid pepsin. Using the two stage technique, closely similar regression lines were obtained for predicting the in vitro digestibility of both grasses and legumes. Use of the pepsin treatment also enabled a less active Basidiomycete cellulase to be used with results very similar to those obtained with the T. viride enzyme. The technique is proposed as a more rapid, convenient and precise method of predicting digestibility than the usual in vitro procedure.  aCellulases  aDigestion  aDry matter content  aForage grasses  aDigestibilidade  aNutrição  aPlanta Forrageira  aTrichoderma Viride1 aHAYWARD, M. V.  tJournal of the Science of Food and Agriculturegv. 26, n. 5, p. 711-718, May, 1975.