01859naa a2200373 a 450000100080000000500110000800800410001902400290006010000180008924501520010726000090025952007870026865000130105565000130106865000140108165000160109565000170111165000110112865000170113965000230115665000100117965000110118965000150120065000150121565000100123065300250124065300160126565300270128165300310130865300240133965300290136370000190139277300740141115227972023-05-10 1974 bl uuuu u00u1 u #d7 a10.1079/bjn197400872DOI1 aWELLER, R. A. aPassage of protozoa and valatile fatty acids from the rumen of the sheep and from a continuous in vitro fermentation system.h[electronic resource] c1974 aI. A procedure for sampling digesta from within the omasal canal of sheep given a variety of roughage diets was used to enable comparison to be made of the composition of effluent from the reticulo-rumen with that of rumen fluid. 2. Concentrations of protozoa in effluents, relative to a soluble marker continuously infused intraruminally, were usually less than 20 yo of corresponding rumen fluid concentrations. It was estimated that the amount of protozoal nitrogen leaving the rumen represented less than 2% of dietary N. 3. Passage of volatile fatty acids (VFA) from the rumen in effluent was less than 75% of that indicated by rumen concentrations. 4. A continuous, in vitro fermentation system was developed, in which outputs of protozoa were comparable with in vivo outputs. aAcetates aChromium aDigestion aFatty acids aFermentation aOmasum aParasitology aRuminant nutrition aSheep aSodium aFisiologia aNutrição aOvino aCarbon radioisotopes aEdetic acid aEquipment and supplies aHydrogen-ion concentration aIn vitro techniques aSpectrophotometry atomic1 aPILGRIM, A. F. tThe British Journal of Nutritiongv. 32, n. 2, p. 341-351, Sep. 1974.