02703naa a2200373 a 450000100080000000500110000800800410001902400280006010000190008824501900010726000090029752015970030665000240190365000230192765000280195065000120197865000130199065000220200365000230202565000170204865000170206565000100208265000160209265000190210865000150212765000100214265000170215265300170216965300150218670000140220170000200221570000160223577300780225115223562025-06-08       1976    bl uuuu     u00u1 u    #d7 a10.1071/bi9760557.2DOI1 aSEAMARK, R. F.  aOxygen uptake, glucose utilization, lactate release and adenine nucleotide content of sheep pvarian follicles in culturebeffect of human chorionic gonadotrophin.h[electronic resource]  c1976  aAbstract: A study has been made of the oxygen uptake, glucose utilization, lactate release and cellular content of adenine nucleotides of isolated sheep ovarian follicles (4-6 min in diameter) maintained in organ culture, and of the effects on these parameters of the addition of human chorionic gonadotrophin (hCG). The mean oxygen consumption of the entire follicles when freshly isolated and of the theca and membrana components was 0-56, 1-08 and 0-05 mumol per milligram wet weight of tissue per hour respectively. About 8 mumol of glucose was taken up and 16 mumol of lactate released per milligram wet weight of follicular tissue per hour during the first 24-h period of culture. This rate reduced by about 30% for each subsequent day of culture, but was significantly increased by the addition of hCG. The mean ATP content of theca and granulosa tissues was 4-6 and 2-8 nmol/mg wet weight of tissue respectively. There was no discernable change in tissue adenine nucleotide content or energy charge ratio during the 3-day culture period, and prolonged exposure to hCG was without effect. Untreated follicles produced both oestrogen and androgens throughout the culture period. The addition of hCG resulted in a transitory stimulation in oestrogen secretion, inhibition of androgen secretion, and a marked and sustained rise in progestin secretion. It is proposed that the increase in glycolytic activity following exposure to hCG may relate to the activation of the granulosa cells coincident with a transference of steroid synthetic capacity from theca interna to membrana granulosa.  aAdenine nucleotides  aCatechol estrogens  aChorionic gonadotropins  aGlucose  aLactates  aOvarian follicles  aOxygen consumption  aPharmacology  aReproduction  aSheep  aBioquímica  aEndocrinologia  aFisiologia  aOvino  aReprodução  aDrug effects  aProgestins1 aAMATO, F.1 aHENDRICKSON, S.1 aMOOR, R. M.  tAustralian Journal Biology Sciencegv. 29, n. 5/6, p. 557-563, Dec. 1976.