02369naa a2200289 a 450000100080000000500110000800800410001910000210006024501020008126000090018352015800019265000210177265000150179365000100180865000100181865000170182865000110184565000120185665000210186865000170188965000150190665300150192165300310193665300150196770000160198277300810199815217512023-08-07       1983    bl uuuu     u00u1 u    #d1 aBILASPURI, G. S.  aBiochemical studies on acid phosphatase in the goat (Capra hircus) testis.h[electronic resource]  c1983  aAbstract: A biochemical study was made to characterize the Acph in the total testicular homogenate of the adult goat (Capra hircus) as well as the different enzymes revealed by acrylamide gel electrophoresis. Total Acph activity was doubled by a two-fold increase in enzyme concentration. A hyperbolic curve of Acph was found with an increase in substrate concentration. V,,and Km values were, respectively, 7.5 uM of p-nitrophenol released/min./mg protein and 2 mM. pH optima were pH 5 (acetate buffer) and pH 4.5 (citrate phosphate buffer). The optimal temperature was 30 oC. The divalent metal ions activated Acph in the decreasing order of Mnz+, Co'+, Mg2+ and Zn2+. Cuz+, NaF and DDVP decreased enzyme activity gradually with increasing concentrations. Formaldehyde and acetone completely inhibited Acph activity, while triton x-100 had no effect. Acrylamide gel electrophoresis of total homogenate with a-naphthyl phosphate revealed three enzymes (I, 11 and 111) of acid phosphatase which varied in their biochemical characteristics. Enzyme I was the most thermo-resistant; at 70 "C the activity of all three enzymes ceased. Metal ions (Mn2+, Mg", Co2+, Znz+), especially Mnz+, markedly activated enzyme III; enzyme I was only slightly activated, and enzyme I1 remained unaffected. Copper (4 mM) markedly inhibited all enzymes. NaF (500 pM) eliminated the activity of enzyme 11 and that of enzymes I and 111 was also markedly decreased. It is concluded, as in other mammalian species studied to date, multiple forms of acid phosphatases exist in the testis of the goat.  aAcid phosphatase  aEnzymology  aGoats  aMales  aReproduction  aTestes  aCaprino  aFosfatase Ácida  aReprodução  aTestículo  aAndrologia  aHydrogen-Ion Concentration  aIsoenzymes1 aDEOL, K. K.  tZentralblatt für Veterinärmedizin. Reihe Agv. 30, n. 7, p. 498-505, 1983.