01452naa a2200241 a 450000100080000000500110000800800410001910000190006024501350007926000090021452007320022365000140095565000100096965000150097965000090099465000140100365000120101765000390102965000120106865000240108070000180110477300880112215202402023-06-20       1971    bl uuuu     u00u1 u    #d1 aCAMERON, C. M.  aImmunological and chemical characteristics of Corynebacterium pseudotuberculosis cell walls and protoplasm.h[electronic resource]  c1971  aSucessive extraction of purified Corynebacterium pseudotuberculosis cell walles with ether: ethanol, cold and hot trichloroacetic acid and crystalline trypsin did not destroy their immunizing potency. Cell walls were not susceptible to the action of lysozyme unless pre-treated with trichloroacetic acid and NaOH. Treatment with NaOH, however, destroved the immunizing properties. Protoplasm also contains immunizing antigen but because of the insolubility of the antigen it could not be purified and characterized. By gel diffusion it was found that cell walls and a protoplasm fraction contain a common antigen. It could not, however, be proved that this antigen is exclusively responsible for inducing a protective immunity.  aCytoplasm  aGoats  aImmunology  aMice  aBactéria  aCaprino  aCorynebacterium Pseudotuberculosis  aDoença  aLinfadenite Caseosa1 aPURDOM, M. R.  tThe Onderstepoort Journal of Veterinary Researchgv. 38, n. 2, p. 83-92, Jun. 1971.