02761naa a2200385 a 450000100080000000500110000800800410001902400450006010000190010524501730012426000090029752016070030665000230191365000130193665000150194965000270196465000130199165000160200465000140202065000190203465000230205365000120207665000180208865000100210665300260211665300310214265300180217365300180219165300120220965300120222165300300223365300280226370000180229177300660230915195232023-12-11       1982    bl uuuu     u00u1 u    #d7 a10.1111/j.1348-0421.1982.tb00266.x.2DOI1 aCOLDITZ, I. G.  aThe role of humoral and cellular mediators in enhanced mammary inflammatory reactions to staphylococcal infection in systemically immunized ewes.h[electronic resource]  c1982  aAbstract: Prior systemic immunization with live Staphylococcus aureus vaccine enhances the early recruitment of neutrophils into nonlactating mammary glands infected with staphylococci. The study investigates the role of humoral and cellular mediators in this phenomenon. Intramammary infusion of bacteria suspended in immune sheep serum did not enhance the inflammatory response to infection in nonimmunized ewes despite the presence of complement in the infused serum. Infusion of complement activated by incubation with zymosan evoked a massive neutrophil influx into mammary secretions by 4 hr after infusion. Hemolytic complement activity was not detected in mammary secretions of immunized or nonimmunized ewes. These findings indicate that, despite the inflammatory effect of complement activation, humoral immune factors did not promote neutrophil migration into infected glands. Mammary glands of systemically immunized ewes stimulated 5 days previously with staphylococcal soluble antigens (SSA) supported larger neutrophil influxes during staphylococcal infection than contralateral glands stimulated with endotoxin 5 days prior to infection. Exudates of SSA-stimulated glands had significantly higher cell concentrations, prior to infection, than endotoxin-stimulated glands; however elevated cell concentrations in endotoxin-stimulated glands of nonimmune ewes did not support enhanced inflammatory responses. These findings suggest that qualitative but not quantitative characteristics of mammary leucocytes influence the inflammatory response to infection in systemically immunized ewes.  aAntibody formation  aEtiology  aImmunology  aMammary gland diseases  aMastitis  aNeutrophils  aPregnancy  aSheep diseases  aSomatic cell count  aDoença  aMicrobiologia  aOvino  aAdministration dosage  aComplement system proteins  aComplications  aEstafilococus  aInfecao  aMastite  aStaphylococcal infections  aStaphylococcal Vaccines1 aWATSON, D. L.  tMicrobiology and Immnologygv. 26, n. 12, p. 1171-1180, 1982.