01529naa a2200277 a 450000100080000000500110000800800410001910000180006024501140007826000090019252007750020165000190097665000130099565000090100865000170101765000100103465000160104465000100106065000170107065000110108765000190109865000150111765300190113270000210115177300790117215195222023-08-02       1975    bl uuuu     u00u1 u    #d1 aWATSON, P. F.  aThe influence of some fractions of egg yolk on the survival of ram spermatozoa at 5oC.h[electronic resource]  c1975  aAbstract: Ram spermatozoa were stored at 5 degrees C in diluents containing various fractions of egg yolk prepared by dialysis, ultrafiltration and ion-exchange chromatography. They survived storage best in the presence of components of egg yolk which were non-dialysable and were not filtered through membranes which retained substances of molecular weight greater than 100 000. The substances isolated in peak B of the ion-exchange chromatogram of whole egg yolk described by Seideman et al. (1969) gave greater protection than those from other fractions from this chromatographic system. These data indicate that the low-density lipoprotein fraction of egg yolk is the most likely source of protection to ram spermatozoa against the effects of storage at 5 degrees C.  aChromatography  aEgg yolk  aRams  aReproduction  aSheep  aSpermatozoa  aOvino  aReprodução  aSêmen  aSobrevivência  aTecnologia  aSemen diluents1 aMARTIN, I. C. A.  tAustralian Journal of Biology Sciencygv. 28, n. 2, p. 145-152, Apr. 1975.