02947naa a2200301 a 450000100080000000500110000800800410001910000190006024500910007926000090017052021100017965000120228965000200230165000260232165000100234765000130235765000140237065000120238465000190239665000240241565300250243965300270246465300280249170000200251970000190253970000160255877300710257415141202014-09-15       1993    bl uuuu     u00u1 u    #d1 aJOHNSON, E. H.  aObservations on goats experimentally infected with Corynebacterium pseudotuberculosis.  c1993  aAbstract: Moxoto goats (n = 17) were experimentally inoculated intradermally with broth culture containing varying numbers of living Corynebacterium pseudotuberculosis and its exotoxin, five goats with broth culture supernatant containing solely crude exotoxin and five goats with washed C. pseudotuberculosis suspended in physiological saline. Three goats inoculated with sterile medium only and four unino- culated goats served as contrais. Compared with controls, Moxoto goats inoculated with broth cul tures of C. pseudotuberculosis and exotoxin, or solely with the crude exotoxin, became acutely ill, with depression, rises in rectal temperature and edema at the site of inoculation. The hematocrit, total serum protein and serum immunoglobulin levels decreased in these animais. Contral goats and goats inoculated with washed bacteria did not exhibit these changes. These results indicate that the intradermal inoculation of goats with washed bacterial cell preparations results in the manifestation of a disease closely resembling naturally occurring caseous Iymphadenitis and, thus, serves as an appropriate challenge system for vaccine studies. Résumé: Les moutons de Moxoto (n=17) ortele piquer avec les mens culture contenant un nombre varie de C. pseudotuberculosis vivante et son exotoxin, 5 moutons avec les meme cultures C. pseudotuberculosis et 5 moutons avec C. pseudotuberculosis baignant dans un liqide physiologique. Apres le controle et la comparison, des moutons de Moxoto pique avec les 2 cultures de C. pseudotuberculosis et exotoxin, ou legerement avec germes, tomberent soudainement malade, avec chutes et augmentation subite de temperature, et un odeme au point de d'inoculation. Les hematocrit au serum de proteines et le niveau de serum immunglobin diminuaient. Les moutons controles et les moutons inocule avec une solution de bacteries ne manifesterent pas ce changement. Le resultat de cette etuide montre que les observations clinicopathologique etaient causees par les exotoxin. Il est doutex que ces effects sont en aucum cas visibles dans les apparitions d'epidermie.  aAbscess  aAnimal diseases  aCaseous lymphadenitis  aGoats  aAbscesso  aBactéria  aCaprino  aDoença animal  aLinfadenite caseosa  aArtificial infection  aExperimental infection  aInfecção experimental1 aVIDAL, C. E. S.1 aSANTA ROSA, J.1 aKASS, P. H.  tSmall Ruminant Research, Amsterdamgv. 12, n. 3, p. 357-369, 1993.