01859naa a2200265 a 450000100080000000500110000800800410001910000290006024500920008926000090018152010090019065000120119965000160121165000130122765000180124065000140125865300430127265300380131565300420135365300270139565300390142270000220146170000200148377300900150310491512010-11-30       2007    bl uuuu     u00u1 u    #d1 aBERTIOLI, S. C. de M. L.  aTargeting and genotyping RGAs in a mapping population of the AA genome of wild Arachis.  c2007  aPeanut is an important legume crop. Although it has high levels of morphological diversity, it lacks genetic variability and sources of disease resistance. The transference of resistance genes from wild species is difficult due to the different ploidy level of the wild and cultivated species. Recently, amphidiploids have been produced that can be used as ‘bridges’ to introgress wild genes. Molecular markers are useful to pyramidize desirable genes and track them through generations of backcrossings. Molecular markers based on Resistance Gene Analogs have improved chances to be present in or linked to resistance gene loci. This study describes the development and genotyping of molecular markers based on resistance gene motifs. Specific primers were designed based on unique sequences of an Arachis RGA dataset. The identity of the amplified polymorphic bands was confirmed by sequencing. These markers were genotyped on a F 2 population that segregates for resistance to biotic stress types.  aArachis  aAgricultura  aAmendoim  aBiotecnologia  aGenética  aAbalho descreve o Primers específicos  aAnálogos a genes de resistência  aGenotipagem de marcadores moleculares  aMarcadores moleculares  aSeleção assistida por marcadores1 aGUIMARÃES, P. M.1 aBERTIOLI, D. J.  tCrop Breeding and Applied Biotechnology, Londrinagv. 7, n. 2, p. 179-185, Mar. 2007.