02410naa a2200241 a 450000100080000000500110000800800410001910000190006024502060007926000090028552016370029465000110193165000090194265000120195165300370196365300100200065300130201065300170202370000170204070000190205770000140207677300780209014912652018-05-24       2008    bl uuuu     u00u1 u    #d1 aMOTA, F. F. da  aBacterial and fungal communities in bulk soil and rhizospheres of aluminum-tolerant and aluminum-sensitive maize (Zea mays L.) lines cultivated in unlimed and limed cerrado soil.h[electronic resource]  c2008  aLiming of acidic soils can prevent aluminum toxicity and improve crop production. Some maize lines show aluminum (AI) tolerance, and exudation of organic acids by roots has been considered to represent ao important mechanism involved in the tolerance. However, there is no information about the impact of liming on the structures of bacterial and fungal communities in Cerrado soil, nor if there are differences between the microbial communities from the rhizospheres of AI-tolerant and AI-sensitive maize lines. This study evaluated the effects of liming on the structure of bacterial and fungal communities in bulk soil and rhizospheres of AI-sensitive and AI-tolerant maize (Zea mays L.) lines cultivated in Cerrado soil by PCR-DGGE, 30 and 90 days after sowing. Bacterial fingerprints revealed that the bacterial communities from rhizospheres were more affected by aluminum stress in soil than by the maize line (AI-sensitive or AI-tolerant). Differences in bacterial communities were also observed over time (30 and 90 days after sowing), and these occurred mainly in the Actinobacteria. Conversely, fungal communities from the rhizosphere were weakly affected either by liming or by the rhizosphere, as observed from the DGGE profiles. Furthermore, only a few differences were observed in the DGGE profiles of the fungal populations during plant development when compared with bacterial communities. Cloning and sequencing of 16S rRNA gene fragments obtained from dominant DGGE bands detected in the bacterial profiles of to be Cerrado bulk soil revealed that Actinomycetales and Rhizobiales were among the dominant ribotypes.  aliming  asoil  aCerrado  aBacterial and fungal communities  aMaize  aPCR-DGGE  aRhizospheres1 aGOMES, E. A.1 aMARRIEL, I. E.1 aPAIVA, E.  tJournal of Microbiology and Biotechnologygv. 18, n. 5, p. 805-814, 2008.