01901naa a2200229 a 450000100080000000500110000800800410001910000200006024501170008026000090019752012210020665300140142765300210144165300350146265300210149770000180151870000210153670000210155770000180157870000190159677300560161514891032018-05-30       2001    bl uuuu     u00u1 u    #d1 aPAPERNIK, L. A.  aPhysiological basis of reduced Al tolerance in ditelosomic lines of Chinese spring wheat.h[electronic resource]  c2001  aAluminium tolerance was assessed in the moderately Al-tolerant wheat (Triticum aestivum) cultivar Chinese Spring and a set of ditelosomic lines derived from Chinese Spring. Three ditelosomic lines lacking chromosome arms 4DL, 5AS and 7AS, respectively, exhibited decreased Al tolerance relative to the euploid parent Chinese Spring based on reduced root growth in Al-containing solutions. The physiological basis of the reduced Al tolerance was investigated. Measurements by inductively coupled argon plasma mass spectroscopy of root apical Al accumulation demonstrated that two of these three lines had a decreased ability to exclude Al from the root apex, the site of Al phytotoxicity. As Al-induced malate exudation has been suggested to be an important physiological mechanism of Al tolerance in wheat, this parameter was quantified and malate exudation was shown to be smaller in all three deletion lines compared with Chinese Spring. These results suggest that the decreased Al tolerance in at least two of the three ditelosomic lines is due to the loss of different genes independently influencing a single Al-tolerance mechanism, rather than to the loss of genes encoding alternative Al-tolerance mechanisms.  aAluminium  aDitelosomic line  aGenetics of aluminum tolerance  aMalate exudation1 aBETHEA, A. S.1 aSINGLETON, T. E.1 aMAGALHAES, J. V.1 aGARVIN, D. F.1 aKOCHIAN, L. V.  tPlanta, New Yorkgv. 212, n. 5/6, p. 829-834, 2001.