00888naa a2200145 a 450000100080000000500110000800800410001910000140006024500780007426000090015252004700016165000130063170000180064477300800066214787142018-07-18       1988    bl uuuu     u00u1 u    #d1 aBRUNE, W.  aParameter evaluation in Michaelis-menten kinetics.h[electronic resource]  c1988  aParameter estimation reliability in enzyme kinetics depends upon the substrate range concentrations under assay. An inappropriate concentration set may lead to spurious values of km and Vmax in the Michaelis-Menten approach. In this paper, the theoretical arguments for a practical criterium concerning the best work range of substrate concentration are discussed on a velocity ratio basis (V1/Vn) as response to the pertinent substrate concentration ratio (S1/Sn).  aQuímica1 aFABRIS, J. D.  tArquivos de Biologia e Tecnologia, Curitibagv. 31, n. 4, p. 527-538, 1988.