01933naa a2200253 a 450000100080000000500110000800800410001902400480006010000210010824502470012926000090037652009830038565000210136865000370138965000250142665000170145165000520146865300200152065300250154070000260156570000160159170000170160777300550162410469902022-05-11       2004    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1002/elps.2004061052DOI1 aFUNES-HUACCA, M.  aSemiquantitative determination of Alicyclobacillus acidoterrestris in orange juice by reverse-transcriptase polymerase chain reaction and capillary electrophoresisblaser induced fluorescence using microchip technology.h[electronic resource]  c2004  aThe semiquantitative detection ofAlicyclobacillus acidoterrrestrisin orange juice byreverse-transcriptase polymerase chain reaction (RT-PCR) with a linear dynamic rangeof 26105– 2 colony forming units (CFU)/mL in terms of cell count is described.Separation, detection, and quantification of the RT-PCR products were accomplishedusing the Agilent 2100 bioanalyzer in conjunction with the DNA 1000 LabChip kit. After0 and 12 h of enrichment, it was possible to generate a linear standard curve betweenthe amount of cells and amplicon concentration of RT-PCR and PCR products. Usingthis method, cell diminution was verified in samples of orange juice treated with a nat-ural inhibitor (Sapindus saponaria), determining the persistence of viable cells. Semi-quantitative RT-PCR using the Agilent 2100 bioanalyzer is a potentially useful approachfor rapidin vitrodetermination ofA. acidoterrestrisand monitoring of inhibitor sus-ceptibility for the orange juice-producing industry.  aAlicyclobacillus  aAlicyclobacillus acidoterrestris  aMicrochip technology  aOrange juice  aReverse transcriptase polymerase chain reaction  aMiniaturization  aReversetranscriptase1 aREGITANO, L. C. de A.1 aMURLLER, O.1 aCARILLHO, E.  tElectrophoresisgv.25, n.21-22, p.3860-3864, 2004.