02583naa a2200217 a 450000100080000000500110000800800410001910000220006024500680008226000090015050000200015952018560017970000210203570000170205670000150207370000170208870000160210570000150212170000140213677302150215014689652006-03-03       2005    bl ---       0--  u    #d1 aNEPOMUCENO, A. L.  aDrought induced gene expression in Brazilian soybean genotypes.  c2005  aResumo: P 6.34.  aBrazil is the second soybean producer in the world, however, drought events frequently cause great reduction in soybean production. Southern Brazilian states, responsible for approximately 40% of Brasil's total soybean production, lost more than 30% production due to drought in the last two years (2004, 2005). Dehydration during drought triggers molecular events that result in physiological and developmental responses. Understanding these mechanisms and how they could be genetically manipulated to improve drought tolerance in commercial crops is one of the big challenges of agricultural researchers. Thus, the objective of this study was the isolation and expression analysis of genes up and down regulated in drought tolerant and drought sensitive Brazilian soybean genotypes during dehydration. Approximately 4.000 expression sequence tags (EST) induced during drought in soybean were cloned and sequenced. Comparison of these sequences with the sequences deposited in data banks allowed the identification of probable gene functions and the categorization of these ESTs. The identified categories include genes involved with cellular protection, transport, cellular structure, cellular division, gene expression regulation, cellular signaling and genes related to biotic and abiotic stress responses. Among these some are known as having differential expression during drought in other plant species, like Trehalose-6-Phosphate synthase, Trehalase synthase, A 1-pirrolina-carboxilato synthase (P5CS), Dehydration Responsive Element Binding Protein (DREB), Aquaporin (PIPl) and Galactinol synthase. Detailed gene expression analysis using real time PCR indicated that a differential expression of some of these genes might be related with differences in physiological and agronomical responses to drought aIso analyzed during the experiments.1 aFARIAS, J. R. B.1 aNEUMAIER, N.1 aTOBITA, S.1 aYAMANAKA, N.1 aBINNECK, E.1 aMOLINA, J.1 aSTOLF, R.  tIn: INTERNATIONAL CONFERENCE ON INTEGRATED APPROACHES TO SUSTAIN AND IMPROVE PLANT PRODUCTION UNDER DROUGHT STRESS, 2., 2005, Rome. InterDrought - II: final program and abstract book. Rome, 2005. Não paginado.