01793naa a2200217 a 450000100080000000500110000800800410001902400430006010000180010324501250012126000090024652011260025565300090138165300250139065300190141565300120143465300110144665300190145770000260147677300730150210468402022-05-05       2004    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1021/jf034467f2DOI1 aVIEIRA, E. C.  aOrthophosphate, phytate, and total phosohorus determination in cerals by flow injection analysis.h[electronic resource]  c2004  aA flow injection spectrophotometric procedure with enzymatic hydrolysis was developed for determination of orthophosphate, phytate and total phosphorus in cereal samples. Phosphorus species were extracted from cereals with 0.05 mol L-1 potassium hydrogen phthalate buffer solution at pH 5.7. Orthophosphate was directly determined in the extracts by molybdenum blue spectrophotometric method. The phytate was hydrolyzed by the enzyme phytase coupled to a solid phase packed into an enzymatic reactor, and the resulting hydrolyzed orthophosphate was also determined by spectrophotometry at 650 nm. After optimization for phosphorus species extraction and enzymatic hydrolysis, a linear calibration graph was obtained up to 196 × 10-6 mol L-1 orthophosphate (P conc) -2.67 + 0.52x, r ) 0.9998). Measurements are characterized by relative standard deviation of 1.6% for a standard of 72 × 10-6 mol L-1 orthophosphate and no baseline drift was observed during 4 h operation periods. It provides 72 measurements per hour, with 2.4 × 10-6 mol L-1 and 7.9 × 10-6 mol L-1 as detection and quantification limits, respectively.  aAcid  aEnzymatic hysrolysis  aImmobilization  aPhytase  aPhytic  aSpectophometry1 aNOGUEIRA, A. R. de A.  tJournal of Agricultural and Food Chemistrygv.52, p.1800-1803, 2004.