02880naa a2200241 a 450000100080000000500110000800800410001910000210006024501510008126000090023230000160024149000370025750001800029452017880047470000160226270000200227870000200229870000200231870000160233870000160235470000220237077302460239214668532007-07-27       2004    bl uuuu     u00u1 u    #d1 aBENEVENTI, M. A.  aMicrosatellite molecular markers associated with selection process of soybean resistant genotypes to Meloidogyne javanica and Heterodera glycines.  c2004  ap. 321-322.  a(Embrapa Soja. Documentos, 228).  aEditado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi.  aNematodes species, especially Meloidogyne javanica and Heterodera glycines cause serious reduction on soybean yield all over the country in Brazil. Development of resistant cultivars is the easiest and most economic control method for the farmers. The molecular marker assisted selection of nematode resistant plants can accelerate the soybean breeding process. In earlier studies, the loci 176 Soy HSP and Satt 114 makers were identified, showing significant correlation with the nematode resistance (M. javanica) of soybean plants. QTL analysis indicated the presence of at least one gene located next to the 176 Soy HSP marker, with a Lod of 27.5. All of the gene extension covered by the Satt 114 and 176 Soy HSP (8.3 cM) markers presented high Lod values, ranging from 22.6 to 27.5. Satt 163 from the G linkage group was used to select resistant plants to Heterodera glycines. Previous studies show that there is a significantly association of this marker with rhg1 resistant gene. Because these markers can effectively help the selection process of plants which have genes of interest, DNA extraction and amplifications of loci 176 Soy HSP and Satt 114, from 104 lines highly resistant and 19 lines highly susceptible, resulting from the cross PI 595099 X BRS 133, were performed. Sixty-four susceptible plants and 42 resistant plants to Heterodera glycines, race 3, resulting from the cross PI 595099 X Coodetec 201, had their DNA extracted and amplified with Satt 163 marker as well. The efficiency of plant selection with resistance to M. javanica based on the loci 176 Soy HSP and Satt 114 was 96,6% and 96,2%, respectively, and the efficiency combined was 100%. The efficiency of plant selection with resistance to Heterodera glycines based on the loci Satt 163 was 81,1%.1 aFUGANTI, R.1 aSILVA, J. F. V.1 aARIAS, C. A. A.1 aMARIN, S. R. R.1 aBINNECK, E.1 aLIMA, C. G.1 aNEPOMUCENO, A. L.  tIn: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004.