02726naa a2200193 a 450000100080000000500110000800800410001910000210006024500890008126000090017030000130017949000370019250001800022952018210040970000170223070000190224770000200226677302460228614668292007-07-27       2004    bl uuuu     u00u1 u    #d1 aBONATO, A. L. V.  aGenetic similarity between soybean cultivars released in Brazil, using AFLP markers.  c2004  a254-255.  a(Embrapa Soja. Documentos, 228).  aEditado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi.  aGenetic similarity between soybean cultivars was studied applying the AFLP (amplified fragment length polymorphism) technique to 317 soybean cultivars released in Brazil from 1962 to 1998. Genetic similarity (GS) coefficients were estimated according to Nei and Li (1979) and the cultivars were clustered by the UPGMA (unweighted pair group method using arithmetic averages) method. The parentage coefficients of 100 cultivars released between 1984 and 1998 were calculated and correlated with the GS coefficient obtained by the markers. AFLP analysis showed approximately 394 bands, 78 of which were polymorphic, with a mean number of 13 bands per primer combination. The GS coefficients varied from 0.17 to 0.97 (x=0.61), with 56.8% of the coefficients being above 0.60 and only 9.7% equal or down than 0.50. The similarity coefficients have remained constant during the last three decades. Bootstrap analysis indicated that the 78 polymorphic markers were sufficient to assess the genetic similarity among the 317 cultivars, due to the relatively low coefficient of variation (7.7%). Dendrogram interpretation was hindered by the large number of cultivars used, but it was possible to detect groups of cultivars formed as expected from their genealogy. Another dendrogram, composed of 63 cultivars, allowed a better interpretation of the groups. Cophenetic correlation was 0.60 and 0.70, respectively, in the 317 and 63 cultivar dendrograms. Parentage coefficients between the 100 cultivars varied from zero to one (x=0.21). However, no significant correlation was detected between the parentage coefficients and the genetic similarity by AFLP (r=0.12). The results showed the efficiency of AFLP markers in large scale studies of genetic similarity and are discussed in relation to the soybean breeding in Brazil.1 aCALVO, E. S.1 aGERALDI, I. O.1 aARIAS, C. A. A.  tIn: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004.