02374naa a2200193 a 450000100080000000500110000800800410001910000280006024501070008826000090019530000160020449000370022050001800025752014320043770000240186970000270189370000140192077302460193414667852007-07-27       2004    bl uuuu     u00u1 u    #d1 aCARPENTIERI-PÍPOLO, V.  aAssociation of isoflavonoids with the imcompatible response of soybean roots to Meloidogyne incognita.  c2004  ap. 166-167.  a(Embrapa Soja. Documentos, 228).  aEditado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi.  aAccumulation of the isoflavonoids has been associated with incompatible response of soybean roots to infection by the root-knot nematode. The soybean isoflavonoids have been proposed to have many effects on host-patogen interaction. The antibiotic accumulated in soybean tissues in response to the patogen attack is the glyceollin which is derived from the isoflavonoid precursor daidzein. Accumulation of isoflavonoids genistin, daidzin and the aglycones genistein and daidzein in roots of soybean following inoculation with race 3 of Meloidogyne incognita was determined in whole-root system by high performance liquid chromatography (HPLC). The roots were harvested from controls and nematode-inoculated seedlings 24 hours, 72 hours and 240 hours after the inoculation. The roots were extracted with ethanol and the extracts were subjected to HPLC. There was no significant defected difference between susceptible cultivar Picket 71 either resistant cultivar FT-Cometa 24 hours after inoculation. Daidzein and genistein were detected 72 hours and 240 hours after the inoculation at the resistant cultivar FT-Cometa. For the susceptible cultivar Picket 71 there was no significative difference for isoflavnoid accumulation between the control or inoculated seedlings. The resistant cultivar FT-Cometa showed maximum concentration of daidzein with range variation from 0.181 to 1.025 mg/100g roots 240 hours after inoculation.1 aMANDARINO, J. M. G.1 aCARRÃO-PANIZZI, M. C.1 aSOUZA, A.  tIn: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004.