03397naa a2200193 a 450000100080000000500110000800800410001910000200006024501240008026000090020430000110021349000370022450001800026152024560044170000200289770000200291770000200293777302460295714667642007-07-27       2004    bl uuuu     u00u1 u    #d1 aFERRACIN, L. M.  aInhibition of Heterodera glycines and Meloidogyne javanica nematoides in soybean by rhizobacteria in greenhouse trials.  c2004  ap. 92.  a(Embrapa Soja. Documentos, 228).  aEditado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi.  aThe incidence and damage of nematodes in soybeans has grown in the last years in Brazil. The present study aimed to evaluate the capacity of rhizobacteria, inoculated in soybean seeds, in reducing the incidence and damage of the root knot nematode (Meloidogyne javanica) and the cyst nematode (Heterodera glycines race 3). Twenty-eight bacterial isolates (most of them Pseudomonas spp.) were screened in greenhouse for the control of cyst nematode and root knot nematode. The bacterial isolates were grown in trypticase soy agar, one tenth of the strength (0.1X TSA), at 28±1°C, for 24 to 48 h. The bacterial cells were harvested, suspended in 0.1M MgSO4 (pH 7,0) and the optical density adjusted for an absorbance of 0.55 at 600 çm. Three seeds of soybean cvs. 'Lee 68' (cyst nematode) or 'BRS 133' (root knot nematode), inoculated with one of the isolates, were sown in pots containing 3 kg of a sterile substrate. For the inoculation, the seeds were immersed in the cell suspension of each isolate or into sterile 0.1M MgSO4 (nonbacterial control). Each treatment was replicated ten times in a completely randomized design. Seven days after sowing, plants were thinned to one per pot and the soil was inoculated with 3,000 eggs of each species of nematode. There was also a nonbacterial control with soil non-inoculated with nematodes. The plants were harvested at 35 days after inoculation with nematodes when shoot and root dry weight and number of nematode eggs (root knot nematode) or females (cyst namatode) were evaluated. The data were analyzed by ANOVA and treatment means were separated by the Tukey's test (a=0.05). Eight isolates significantly inhibited the cyst nematode multiplication. The highest inhibition (56%) was observed when the seeds were inoculated with the isolate LW 2301. Although many isolates inhibited the root knot nematode multiplication, none of them were significantly different from the nonbacterial control with nematode. The highest inhibition (72%) was observed with the inoculation of isolate P 70. Some isolates, inversely, stimulated the multiplication; the highest stimulation (132%) was observed with isolate P 61. This event shows, once more, the complexity of ecological interactions in the rhizosphere. Shoot and root weight were not significantly affected, when compared to the nonbacterial control with nematodes, in either experiments. The best isolates are currently being tested in field trials.1 aSILVA, J. F. V.1 aCATTELAN, A. J.1 aBETTI, A. F. F.  tIn: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004.