02196naa a2200253 a 450000100080000000500110000800800410001910000200006024501620008026000090024252014090025165000230166065000160168365000230169965000130172265000230173565000250175865000170178365000090180070000240180970000200183370000210185377300680187414632662024-03-28       2001    bl uuuu     u00u1 u    #d1 aSILVA, J. F. V.  aIdentificação de marcadores moleculares de microssatélites associados à resistência de genótipos de soja a Meloidogyne javanica.h[electronic resource]  c2001  aTwenty loci of simple sequence repeats (SSR) were used to amplify the DNA of 120 F2 plants of the soyabean crosses BRS 133 (M. javanica-susceptible) x Coodetec 201 (resistant) and BRS 133 x PI 595099 (resistant). The loci are located close to some quantitative trait loci (QTLs) on the linkage group D1b+W and F. Each of the F2 (120) and F3 (120 families) generations and the parents (60 of each) were screened in a greenhouse for resistance to gall formation. The plants were grown in plastic tubes (4.5 cm in diameter x 19 cm length) filled with methyl bromide-fumigated soil amended with sand. The seedlings were individually inoculated with 3000 nematode eggs and evaluated by counting the root galls 30 days later. Analysis of variance indicated significant marker/nematode galls association for Satt 266 and Sat 133 (BRS133 x Coodetec 201) and HSP 176 (BRS 133 x PI 595099). This data corroborates the existence of QTLs conferring resistance to M. javanica located close to RFLP markers A725 on linkage-group D1b+W, and A806D and A186D/A757V on linkage-group F. However, the loci detected here did not explain much of the total phenotypic variation of the number of galls. Thus, further studies dealing with the evaluation of SSR loci at the same genomic location will be necessary until these markers can be fully applied to assisted selection of soyabean genotypes with resistance to M. javanica.  aGenetic resistance  aMeloidogyne  aMolecular genetics  aSoybeans  aMarcador Molecular  aMeloidogyne Javanica  aResistência  aSoja1 aFERRAZ, L. C. B. C.1 aARIAS, C. A. A.1 aABDELNOOR, R. V.  tNematologia Brasileira, Brasíliagv. 25, n. 1, p. 79-83, 2001.