02263naa a2200337 a 450000100080000000500110000800800410001910000200006024501880008026000090026852013180027765000190159565000110161465000220162565000130164765000120166065000290167265000160170165000170171765000150173465000140174965000090176365300110177265300140178365300160179765300110181370000180182470000210184270000160186377300460187914588632023-08-31       1996    bl uuuu     u00u1 u    #d1 aNISHI, C. Y. M.  aMorphological, physiological and genetic characterization of two new Bradyrhizobium strains recently recommended as brazilian commercial inoculants for soybean.h[electronic resource]  c1996  aLarge scale soybean inoculation in Brazil started in 1960 and, since then, several strains from other countries were introduced into the Brazilian soil. Since 1992, two new strains have been used as Brazilian inoculants, CPAC 7 AND CPAC 15, which are natural variants of CB 1809 (recieved from Australian in 1966) and SEMIA 566 (isolated from a North American inoculant in 1966), respectively, and were obtained in a selection program for higher efficiency of N2 fixation and competitiveness. Analyses of morphological and physiological parameters, particularly the ones that differentiate the species B. japonicum and B. elkanii, indicated that CB 1809 and the variant CPAC 7 show the characteristics of B. japonicum, while SEMIA 566 and CPAC 15 fit into the species B. elkanii. Differences between parental and variant strains included also several profiles in RAPD analysis. Increases in nodulation, nodule occupancy and yield due to inoculation were observed even in a soil containing 2.2 x 105 cells/g of soil. The evaluation of nodule occupancy is essential to confirm the benefits of inoculation and, since the newly-recommended strains belong to serogroups already established in most Brazilian soils, the RAPD technique seems to be a suitable technique to differentiate the parental from variant strains.  aBradyrhizobium  aBrazil  aNitrogen fixation  aSoybeans  aEstirpe  aFixação de Nitrogênio  aGlycine Max  aInoculação  aInoculante  aRhizobium  aSoja  aBrasil  aInoculant  aInoculation  aStrain1 aBODDEY, L. H.1 aVARGAS, M. A. T.1 aHUNGRIA, M.  tSymbiosisgv. 20, n. 2, p. 147-162, 1996.