01641naa a2200229 a 450000100080000000500110000800800410001910000170006024501330007726000090021052009690021965000130118865000110120165300190121265300240123165300250125565300130128070000260129370000200131970000190133977300530135810449022022-01-04       2000    bl uuuu     u00u1 u    #d1 aSILVA, F. V.  aDetermination of total, volatile and acid detergent insoluble nitrogen in silage by sequential injection.h[electronic resource]  c2000  aThis work proposes a sequential injection procedure to determine the total, volatile and acid detergent insoluble nitrogencontent in silage. An ammonium tubular selective electrode coupled to a sequential injection manifold was used asdetector to accomplish the potentiometric determinations made after on-line ammonia diffusion in alkaline mediumthrough a Teflon membrane and NH3/NH4+conversion. Different sample treatments, taking into account the species to beanalyzed, were adopted to apply the proposed procedure to silage samples. An analytical frequency of 30 samples h?1anda detection limit of 3.0 mg N l?1were observed when 300 ?l of 3.0 mol l?1NaOH solution and a sample volume of 150 ?lwere employed. The use of the proposed procedure allowed the automation of the determinations, low sample andreagent consumption and small waste production, emphasizing the versatility and potentiality of the flow methods whenapplied to animal nutrition laboratories.  anitrogen  asilage  aAcid detergent  aDetermination total  aSequential injection  aVolatile1 aNOGUEIRA, A. R. de A.1 aSOUZA, G. B. de1 aCRUZ, G. M. da  tAnalytical Sciencesgv.16, p.361-364, apr. 2000.