02038naa a2200277 a 450000100080000000500110000800800410001910000190006024500570007926000090013652013080014565000240145365000130147765000150149065300130150565300140151865300100153265300140154270000250155670000240158170000310160570000180163670000190165470000170167377300700169014159232005-12-19       2002    bl ---       0--  u    #d1 aBARRETO, M. L.  aDetection of Mycoplasma pulmonis in laboratory rats.  c2002  aThis work was conducted on rats in two premises located in Niterói and Rio de Janeiro, Brazil. One is classified as conventional controlled and the other, conventional. The objective of the present study was to detect the presence of Mvcoplama pulmonis in animals with symptoms of respiratory disease and low reproductive performance. In the conventional controlled premises, 16 rats of Wistar-Furth strain were necropsjed while in the conventional premises necropsy was performed on 12 rats of Hooded Lister strail1. The clil1ical samples of lungs, trachea, oropharynx, middle ear, uterus and ovaries were subjected to culturil1g while the sera were tested for antibody detection. From 28 rats, 57.14% ( 16/28) were culture positive for M. pulmonis, beil1g 81.25% (13/16) from the conventional controlled premises, and 25.00% (3/12) from the conventional premises, The ELISA test was carried out in 20 animals of  both colonies. In the conventional col1trolled premises, 92.86% ( 13/14) were positive for M pulmonis, and 7.14% ( 1/14) were suspicious, while in the conventional premises, 100% (6/6) of the samples were positive. The results confirmed that M. plllmol1is was the etiologic agent of the disease that affected the rats under study, and that the ELISA positivity rated higher than culture.  aMycoplasma pulmonis  aserology  aIsolamento  aDiagnose  aDiagnosis  aRatos  aSorologia1 aNASCIMENTO, E. R. do1 aCAMPOS, C. A. de M.1 aNASCIMENTO, M. da G. F. do1 aLIGNON, G. B.1 aLIRA, M. L. F.1 aSILVA, R. G.  tBrazilian Journal of Microbiology, São Paulogn. 33, p. 262-266.