01556nam a2200289 a 450000100080000000500110000800800410001910000240006024501260008426000390021030000160024950000180026552007770028365000170106065000130107765000100109065000130110065000190111365000220113265000120115465000100116665000140117665000170119065000250120765000210123265300130125314025682025-01-08       1993    bl uuuu  m   00u1 u    #d1 aDUARTE, M. de L. R.  aToxic metabolites of Nectria haematococca f. sp. piperis and their role in pathogenesis on black pepper, Piper nigrum, L.  aLondon: University of Londonc1993  a213 p.cil.  aPh.D. Thesis.  aThe aim of the present study was to establish a selecting resistant black pepper plants from susceptible germplasm using as selective agent a mixture of toxins produced by the pathogen. The route to this objective is: To establish methods of production of fungal culture, host callus and cell suspension cultures; To study the reaction of the whole plant and callus to pathogen colonization; To investigate the reaction of callus and cell suspension when growing in contact with toxic culture filtrate; To elucidate the role played pathogen-produced toxin in pathogenesis; To purify toxin(s) produced the pathogen; To select resistant sectors from callus of different cultivars through passage in toxic culture medium; To try to regenerate black pepper plants from callus.  ablack pepper  abreeding  afungi  agenetics  atissue culture  aCultura de Tecido  aDoença  aFungo  aGenética  aMelhoramento  aNectria Haematococca  aPimenta do Reino  aDiseases