01746naa a2200277 a 450000100080000000500110000800800410001902400580006010000260011824501790014426000090032352008640033265000230119665000220121965000140124165000120125565000090126765000090127670000150128570000180130070000200131870000220133870000200136070000200138077300680140013363792021-09-20       1999    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1046/j.1462-2920.1999.00047.x2DOI1 aCOUTINHO, H. L. da C.  aMolecular evidence for shifts in polysaccharide composition associated with adaptation of soybean Bradyrhizobium strains to the Brazilian Cerrado soilsh[electronic resource]  c1999  aPyrolysis mass spectrometry (PyMS) and DNA fingerprinting (RAPD and RSa hybridization) were used to characterize soybean inoculant strains and root nodule isolates of bradyrhizobia from the Brazilian Cerrado soils. Most isolates were shown to be derived from the inoculant strains on the basis of genotype comparisons by DNA fingerprinting. Phenotypic analysis (using PyMS) of the strains and separately of the polysaccharides derived from them showed that the nodule isolates differed from the parental strains, suggesting adaptation to the Cerrado soil environment. The extent of the differences between the derivatives and inoculant strains was similar for comparisons made on the basis of whole-cell preparations or from the isolated polysaccharides, indicating that the adaptation was caused by changes in the composition of the polysaccharides produced.  aDNA fingerprinting  aMass spectrometry  aPyrolysis  aCerrado  aSoja  aSolo1 aKAY, H. E.1 aMANFIO, G. P.1 aNEVES, M. C. P.1 aRIBEIRO, J. R. A.1 aRUMJANEK, N. G.1 aBERINGER, J. E.  tEnvironmental Microbiologygv. 1, n. 5, p. 401-408, Sept. 1999.