01876naa a2200337 a 450000100080000000500110000800800410001902400580006010000230011824502140014126000090035552008310036465000200119565000090121565000180122465000080124265000170125065000140126765000090128165300130129065300130130365300160131665300140133265300120134670000260135870000210138470000210140570000210142670000180144777300730146513353642023-08-25       1999    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1046/j.1365-2672.1999.00480.x2DOI1 aOLIVEIRA, V. M. de  aDiscrimination of Rhizobium tropici and R. leguminosarum strains by PCR-specific amplification of 16S-23S rDNA spacer region fragments and denaturing gradient gel electrophoresis (DGGE).h[electronic resource]  c1999  aWith the aim of detecting Rhizobium species directly in the environment, specific PCR primers for Rh, tropici and Rh, leguminosarum were designed on the basis of sequence analysis of 16S-23S rDNA spacer regions of several Rh. tropici, Rh. leguminosarum and Agrobacterium rhizogenes strains. Primer specificity was checked by comparison with available rDNA spacer sequences in databases, and by PCR using DNA from target and reference strains. Sequence polymorphisms of rDNA spacer fragments among strains of the same species were detected by denaturing gradient gel electrophoresis (DGGE). The specific PCR primers designed in the study could be applied to evaluate the diversity of Rh. tropici and Rh. leguminosarum by analysing the polymorphisms of 16S-23S spacer rDNA amplified from either whole-cell or soil-extracted DNA.  aelectrophoresis  asoil  aBase de Dados  aDNA  aEletroforese  aRhizobium  aSolo  aAnalysis  aDatabase  aDiversidade  aDiversity  atropici1 aCOUTINHO, H. L. da C.1 aSOBRAL, B. W. S.1 aGUIMARAES, C. T.1 aELSAS, J. D. van1 aMANFIO, G. P.  tLetters in Applied Microbiologygv. 28, N. 2, p. 137-141, Feb. 1999.