02805naa a2200409 a 450000100080000000500110000800800410001910000150006024501900007526000090026550000110027452015940028565000110187965000130189065000170190365000200192065000220194065000350196265000160199765000230201365000280203665000240206465000250208865300170211365300110213065300290214165300130217065300180218365300090220170000170221070000180222770000170224570000200226270000210228270000240230377300680232713189951998-07-10       1997    bl uuuu     u00u1 u    #d1 aKELEMU, S.  aBiodeversity, epidemiology and virulence of colletotrichum gloeosporioides. I.Genetic and patrhogenica diversituy in colletotrichum gloesporioides isolates from Stylosanthes guianensis.  c1997  aCNPGC.  aAnthracnose, caused by Colletotrichum gloeosporioides, is the most important and widespred disease of Stylosanthes, a diverse tropical and subtropical forage legume naturally distributed in central aud South America. This paper compares the genetic and pathogenic diversity of  45 isolates, which originated from S. guianensis genotypes. Published information on the pathgenic diversity of these isolates on a set of 12 S. guianensis was used. The amount of genetic diversity was measured at molecular level by polymerase chain reaction amplifications of DNA with 9 arbitrary primers of 10 bases each using the Random Amplified Polymorphic DNA  (RAPD) markers. The amplifications revealed scorable polymorphism among the isolates, and a total of 80 band positiouns were scored. Using Ward's method (N = 6) of statistical analysis, the isolates were separated into 6 clusters. Generally, isolates were clustered together by their geagraphic origin aud/or their original host genotype. Isolates from Carimagua, Colombia,  a savanua ecosystem and a loug-time Stylosanthes. breeding and selection site, exhibited a relatively wider range of genetic diversity than those from  a newly opened trial site in the Amazon basin of Colombia. No strict correlation existed between genetic diversity, as measured by RAPD, and differential virulence, as defined by the pathotype. lsolates of the same pathotype/RAPD grouping often originated from the same host genotype and/or geographical locality. This inforrnation will be useful in deternining future sampling strategies for the pathogen population.  aBrazil  aColombia  aepidemiology  agenetic markers  agenetic variation  aColletotrichum Gloeosporioides  aEstilosante  aMarcador Genético  aStylosanthes Guianensis  aStylosanthes Scabra  aVariação Genética  aAnthracnosis  aBrasil  aEpidemiologia Antracnose  aMineirao  aPathogenecity  aRAPD1 aBADEL, J. L.1 aMORENO, C. X.1 aMILES, J. W.1 aCHAKRABORTY, S.1 aFERNANDES, C. D.1 aCHARCAR, M. J. D`A.  tTropical Grasslands, Brisbanegv.31, n.5, p.387-392, Nov. 1997.