01791naa a2200193 a 450000100080000000500110000800800410001910000210006024500660008126000090014752012950015665000150145165000210146665000140148765000230150165000220152470000150154677300360156112798482025-03-27       1975    bl uuuu     u00u1 u    #d1 aCRESSWELL, R. J.  aOrgan culture of Eucalyptus grandis L.h[electronic resource]  c1975  aOrgan culture of nodes of Eucalyptus grandis permitted the rooting of nodes from much beyond No. 14, the highest node from which rooting has been described so far, namely up to node No. 80, the eotyledonary node being No. 1. Plantlets which were obtained from node 50 have been successfully transferred to the field. The classical methods of vegetative propagation are n o t successful when used on adult-phase trees of most species of Eucalyptus. B y the time t h a t these trees have reached the age a n d size at which t h e y can be evaluated b y foresters a n d geneticists, t h e y have passed the stage at which t h e y can be propagated vege- tatively. P a t o n et al. (1970) a n d Nicholls st al. (1970) have isolated from adult tissue of E. grandis, three rooting inhibitors the c o n c e n t r a t i o n of which irmreases with the decreased rooting ability of stem cuttings. These authors s t a t e 4 h a t in E. grandis there is a complete loss of rooting ability when the base of the c u t t i n g is t a k e n above node No. 14 (The cotyledonary node being node No. 1). The techniques of organ culture in vitro were adopted in an a t t e m p t at ob- t a i n i n g rooting a n d t h e n plantlets which can be transferred to the field, from nodes above No. 14 in E. grandis.  aEucalyptus  aCultura In Vitro  aEucalipto  aEucalyptus Grandis  aMicropropagação1 aNITSCH, C.  tPlantagv. 125, p. 87-90, 1975.