01500naa a2200169 a 450000100080000000500110000800800410001910000140006024501150007426000090018952009980019865000150119665000210121165000120123265300160124477300700126012798032025-03-18       1976    bl uuuu     u00u1 u    #d1 aHU, C. Y.  aLight-mediated inhibition of in vitro development of rudimentary embryos of Ilex opaca.h[electronic resource]  c1976  aThe mature seeds of Ilex opaca Ait. contain rudimentary heart-shaped embryos. When excised embryos of cv. Farage were grown in vitro with a 16-hr photoperiod, only 20 % reached germination size after 13-day incubation, while 75% of the embryos reached the same stage when incubated in darkness. This light inhibitory effect increased with the length of daily light exposure as the daily photoperiod reached 4 hr. Nearly 50% growth reduction resulted as the cultures were preincubated in light continuously for 2 days before a 10-day dark incubation. After 4 days of light incubation the inhibitory effect could no longer be reversed by the subsequent dark incubation. Once the heart-shaped embryos started to grow in darkness they become progressively insensitive to subsequent light inhibition. After a 6-day initial dark incubation the embryos become immune from the inhibitory effect of light. This light inhibitory effect on in vitro embryo growth was universal in all 11 tested cultivars.  aIlex opaca  aCultura In Vitro  aSemente  aDevelopment  tAmerican Journal of Botany, Bronxgv. 63, n. 5, p. 651-656, 1976.