01657naa a2200181 a 450000100080000000500110000800800410001910000220006024500850008226000090016752011530017665000150132965000210134465000140136570000180137970000180139777300600141512792412025-09-02       1977    bl uuuu     u00u1 u    #d1 aFOSSARD, R. A. de  aThe organ culture of nodes of four species of Eucalyptus.h[electronic resource]  c1977  aTwo approaches are being used to clonally-propagateEucalyptus. One is to culture nodes of adult trees and the second is to induce coppice formation from the base of adult trees and to culture this juvenile material. Work with E. ficifoli a (de Fossard and Bourne 1976) led the culture of nodes from 36 year old trees with the formation of healthy shoots on a medium with six auxins. Approximately three times more growth of the buds was obtained when 10μM IBA was used as the sole auxin source and best growth was obtained with 100µM Fe in place of 50uM Fe in the medium. Although all seedling nodal cultures and subcultures were induced to form roots on cytokinin free media with 10µM IBA, so far adult nodal cultures have not been induced to form roots. Nodes from coppiced E. grand i s made good shoot growth and some root growth with only a little callus on a cytokinin free medium selected from the Broad Spectrum Experiment (de Fossard, Myint and Lee, 1974), whereas they made very strong root growth with abundant callus on the medium developed by Cresswell and Nitsch (1975). Excellent plantlets were obtained by combining the two media.  aEucalyptus  aCultura In Vitro  aEucalipto1 aBARKER, P. K.1 aBOURNE, R. A.  tActa Horticulturae, The Haguegn. 78, p. 157-165, 1977.