01541naa a2200361 a 450000100080000000500110000800800410001910000170006024500890007726000090016652005570017565000180073265000150075065000220076565000210078765000120080865000100082065000190083065000270084965000220087665000210089865000260091965000220094565300190096765300080098665300080099465300280100265300220103070000230105270000140107570000130108977300770110212786762024-05-22 1987 bl uuuu u00u1 u #d1 aHISAJIMA, S. aMass propagation of Giant Ipil through seed culture in vitro.h[electronic resource] c1987 aIn Leucaena leucocephala, multiple shoot buds were induced from germinating seeds on medium with benzylaminopurine [benzyladenine] (BAP) at 50-150 5M in vitro. Shoots were successively multiplied 3-4 times every 4 weeks in the medium containing 0.5 5M BAP and 0.025 5M IBA. It is calculated that about 1 600 000 shoots could be obtained from a single shoot in a year by this technique. Plant regeneration was achieved by rooting from single shoots. The rooting rate was 70% at 5 5M IAA. Some of the control cultures (without auxin) also produced roots. abenzyladenine acytokinins agrowth regulators amicropropagation arooting aseeds atissue culture avegetative propagation aCultura de Tecido aCultura In Vitro aLeucaena Leucocephala aMicropropagação aFodder legumes aIAA aIBA aPlant growth regulators aPropagacao massal1 aCHONGPRADITNUM, P.1 aOSAKA, J.1 aARAI, Y. tJapanese Journal of Tropical Agriculturegv. 31, n. 4, p. 249-251, 1987.